ano9a 7 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001109835 · chr25

80 Single Exon Specific

FWD 5' AGAAGGGCTCCAAGAAAGCC 3'

REV 5' GGGATGGACCTGCTTCAACA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 119 bp

Single-exon gene (no intron)

NM_001109835 · chr25

80 Single Exon Specific

FWD 5' AGAGAGATCCAGACCGCTGT 3'

REV 5' GTGTACTTGGTGACGGCCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 92 bp

Single-exon gene (no intron)

NM_001109835 · chr25

80 Single Exon Specific

FWD 5' CTCCAAGAAAGCCGTCACCA 3'

REV 5' GGGATGGACCTGCTTCAACA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Single-exon gene (no intron)

NM_001109835 · chr25

80 Single Exon Specific

FWD 5' AGAAAGCCGTCACCAAGACC 3'

REV 5' GGGATGGACCTGCTTCAACA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 107 bp

Single-exon gene (no intron)

NM_001109835 · chr25

79 Single Exon Specific

FWD 5' ACGACATCTTCGAGCGCATC 3'

REV 5' ACAGCGGTCTGGATCTCTCT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 94 bp

Single-exon gene (no intron)

NM_001109835 · chr25

79 Single Exon Specific

FWD 5' CCCAAGAAGGGCTCCAAGAA 3'

REV 5' GGGATGGACCTGCTTCAACA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 123 bp

Single-exon gene (no intron)

NM_001109835 · chr25

78 Single Exon Specific

FWD 5' TGTTGAAGCAGGTCCATCCC 3'

REV 5' TGGTGGAGCGCTTGTTGTAG 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 135 bp

Single-exon gene (no intron)