ap4s1 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001003826 · chr17

117 Specific

FWD 5' CGGAGGAAGGAAGAGTGCTC 3'

REV 5' GCACCTCCACAAAGTTGTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 60%

Product 148 bp

Spans 2 introns

NM_001003826 · chr17

114 Specific

FWD 5' GGAGGAAGGAAGAGTGCTCA 3'

REV 5' GCACCTCCACAAAGTTGTGC 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001003826 · chr17

111 Specific

FWD 5' GGAGGAAGGAAGAGTGCTCA 3'

REV 5' CCTCCACAAAGTTGTGCACA 3'

Fwd Tm 60.8°C

Rev Tm 60.5°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001003826 · chr17

111 Specific

FWD 5' GGAGGAAGGAAGAGTGCTCA 3'

REV 5' ACCTCCACAAAGTTGTGCAC 3'

Fwd Tm 60.8°C

Rev Tm 60.5°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001003826 · chr17

111 Specific

FWD 5' GGAGGAAGGAAGAGTGCTCA 3'

REV 5' CACCTCCACAAAGTTGTGCA 3'

Fwd Tm 60.8°C

Rev Tm 60.5°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001003826 · chr17

110 Specific

FWD 5' GGAGGAAGGAAGAGTGCTCA 3'

REV 5' AGCACCTCCACAAAGTTGTG 3'

Fwd Tm 60.8°C

Rev Tm 60.3°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001003826 · chr17

110 Specific

FWD 5' CCGGAGGAAGGAAGAGTGCT 3'

REV 5' GCACCTCCACAAAGTTGTGC 3'

Fwd Tm 63.4°C

Rev Tm 61.8°C

GC 60%

Product 149 bp

Spans 2 introns

NM_001003826 · chr17

109 Specific

FWD 5' GCACAACTTTGTGGAGGTGC 3'

REV 5' GTCTCCACGATGTGTCCGTT 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001003826 · chr17

109 Specific

FWD 5' GCACAACTTTGTGGAGGTGC 3'

REV 5' CACGATGTGTCCGTTCAGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001003826 · chr17

109 Specific

FWD 5' GCACAACTTTGTGGAGGTGC 3'

REV 5' TCCACGATGTGTCCGTTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 111 bp

Spans 1 intron