apela 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001297547 · chr1_KZ115011v1_alt

109 Specific

FWD 5' CTGTATCTGCTGCTGCTGCT 3'

REV 5' GGGCAGTTGTGTCTGCGATA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 113 bp

Spans 1 intron

NM_001297547 · chr1

109 Specific

FWD 5' CTGTATCTGCTGCTGCTGCT 3'

REV 5' GGGCAGTTGTGTCTGCGATA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 113 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

109 Specific

FWD 5' CTGTATCTGCTGCTGCTGCT 3'

REV 5' TAGACAGCGTTTCTTCGGGC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001297547 · chr1

109 Specific

FWD 5' CTGTATCTGCTGCTGCTGCT 3'

REV 5' TAGACAGCGTTTCTTCGGGC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

109 Specific

FWD 5' GCCCGAAGAAACGCTGTCTA 3'

REV 5' AACAGTCCTCGGCACATCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001297547 · chr1

109 Specific

FWD 5' GCCCGAAGAAACGCTGTCTA 3'

REV 5' AACAGTCCTCGGCACATCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

109 Specific

FWD 5' CTGTATCTGCTGCTGCTGCT 3'

REV 5' TCTTCGGGCAGTTGTGTCTG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001297547 · chr1

109 Specific

FWD 5' CTGTATCTGCTGCTGCTGCT 3'

REV 5' TCTTCGGGCAGTTGTGTCTG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

108 Specific

FWD 5' GGTCCTCATCAGCGCAGATA 3'

REV 5' CTTGCCCGGAGCATCATAAA 3'

Fwd Tm 61.1°C

Rev Tm 60.0°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001297547 · chr1

108 Specific

FWD 5' GGTCCTCATCAGCGCAGATA 3'

REV 5' CTTGCCCGGAGCATCATAAA 3'

Fwd Tm 61.1°C

Rev Tm 60.0°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001297547 · chr1_KZ115011v1_alt

108 Specific

FWD 5' GAGATTCTTCCACCCGCTGT 3'

REV 5' TCTTCGGGCAGTTGTGTCTG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001297547 · chr1

108 Specific

FWD 5' GAGATTCTTCCACCCGCTGT 3'

REV 5' TCTTCGGGCAGTTGTGTCTG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

108 Specific

FWD 5' TGAGATTCTTCCACCCGCTG 3'

REV 5' TCTTCGGGCAGTTGTGTCTG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001297547 · chr1

108 Specific

FWD 5' TGAGATTCTTCCACCCGCTG 3'

REV 5' TCTTCGGGCAGTTGTGTCTG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

108 Specific

FWD 5' CACCCTGAGCATCACCATGA 3'

REV 5' GGGCAGTTGTGTCTGCGATA 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

108 Specific

FWD 5' GAGATTCTTCCACCCGCTGT 3'

REV 5' TAGACAGCGTTTCTTCGGGC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001297547 · chr1_KZ115011v1_alt

108 Specific

FWD 5' GCCCGAAGAAACGCTGTCTA 3'

REV 5' GTCCTCGGCACATCTGACAT 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001297547 · chr1

108 Specific

FWD 5' CACCCTGAGCATCACCATGA 3'

REV 5' GGGCAGTTGTGTCTGCGATA 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001297547 · chr1

108 Specific

FWD 5' GAGATTCTTCCACCCGCTGT 3'

REV 5' TAGACAGCGTTTCTTCGGGC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001297547 · chr1

108 Specific

FWD 5' GCCCGAAGAAACGCTGTCTA 3'

REV 5' GTCCTCGGCACATCTGACAT 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 143 bp

Spans 1 intron