aph1b 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200115 · chr7_KZ115231v1_alt

117 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' ATGAAGCCAAAGCCCAGACC 3'

Fwd Tm 61.9°C

Rev Tm 62.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_200115 · chr7

117 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' ATGAAGCCAAAGCCCAGACC 3'

Fwd Tm 61.9°C

Rev Tm 62.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

116 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' CATGAAGCCAAAGCCCAGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_200115 · chr7

116 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' CATGAAGCCAAAGCCCAGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

115 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' CTCATGAAGCCAAAGCCCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.0°C

GC 55%

Product 147 bp

Spans 2 introns

NM_200115 · chr7

115 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' CTCATGAAGCCAAAGCCCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.0°C

GC 55%

Product 147 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

115 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' TCATGAAGCCAAAGCCCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 146 bp

Spans 2 introns

NM_200115 · chr7

115 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' TCATGAAGCCAAAGCCCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 146 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

114 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' GCTCATGAAGCCAAAGCCCA 3'

Fwd Tm 61.9°C

Rev Tm 63.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_200115 · chr7

114 Specific

FWD 5' CCTGCTTCAGGAGGCTTTCA 3'

REV 5' GCTCATGAAGCCAAAGCCCA 3'

Fwd Tm 61.9°C

Rev Tm 63.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

113 Specific

FWD 5' CTGCTTCAGGAGGCTTTCAG 3'

REV 5' CTCATGAAGCCAAAGCCCAG 3'

Fwd Tm 60.3°C

Rev Tm 61.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_200115 · chr7

113 Specific

FWD 5' CTGCTTCAGGAGGCTTTCAG 3'

REV 5' CTCATGAAGCCAAAGCCCAG 3'

Fwd Tm 60.3°C

Rev Tm 61.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

113 Specific

FWD 5' TGCTTCAGGAGGCTTTCAGA 3'

REV 5' CTCATGAAGCCAAAGCCCAG 3'

Fwd Tm 60.8°C

Rev Tm 61.0°C

GC 50%

Product 145 bp

Spans 2 introns

NM_200115 · chr7

113 Specific

FWD 5' TGCTTCAGGAGGCTTTCAGA 3'

REV 5' CTCATGAAGCCAAAGCCCAG 3'

Fwd Tm 60.8°C

Rev Tm 61.0°C

GC 50%

Product 145 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

112 Specific

FWD 5' CTGCTTCAGGAGGCTTTCAG 3'

REV 5' CATGAAGCCAAAGCCCAGAC 3'

Fwd Tm 60.3°C

Rev Tm 61.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_200115 · chr7

112 Specific

FWD 5' CTGCTTCAGGAGGCTTTCAG 3'

REV 5' CATGAAGCCAAAGCCCAGAC 3'

Fwd Tm 60.3°C

Rev Tm 61.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

112 Specific

FWD 5' TGCTTCAGGAGGCTTTCAGA 3'

REV 5' CATGAAGCCAAAGCCCAGAC 3'

Fwd Tm 60.8°C

Rev Tm 61.2°C

GC 50%

Product 143 bp

Spans 2 introns

NM_200115 · chr7

112 Specific

FWD 5' TGCTTCAGGAGGCTTTCAGA 3'

REV 5' CATGAAGCCAAAGCCCAGAC 3'

Fwd Tm 60.8°C

Rev Tm 61.2°C

GC 50%

Product 143 bp

Spans 2 introns

NM_200115 · chr7_KZ115231v1_alt

112 Specific

FWD 5' TGCTTCAGGAGGCTTTCAGA 3'

REV 5' CACCGCTCATGAAGCCAAAG 3'

Fwd Tm 60.8°C

Rev Tm 61.4°C

GC 50%

Product 150 bp

Spans 2 introns

NM_200115 · chr7

112 Specific

FWD 5' TGCTTCAGGAGGCTTTCAGA 3'

REV 5' CACCGCTCATGAAGCCAAAG 3'

Fwd Tm 60.8°C

Rev Tm 61.4°C

GC 50%

Product 150 bp

Spans 2 introns