apnl 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001293671 · chr3

108 Specific

FWD 5' AACGCACCGTTACTGAGCAG 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001293671 · chr3

108 Specific

FWD 5' GAACGCACCGTTACTGAGCA 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001293671 · chr3

108 Specific

FWD 5' AGCACACACAGAGTCCCTCT 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001293671 · chr3

108 Specific

FWD 5' ACCGTTACTGAGCAGCACAC 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.2°C

Rev Tm 62.5°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001293671 · chr3

108 Specific

FWD 5' CACCGTTACTGAGCAGCACA 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.2°C

Rev Tm 62.5°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001293671 · chr3

108 Specific

FWD 5' CCGTTACTGAGCAGCACACA 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.2°C

Rev Tm 62.5°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001293671 · chr3

108 Specific

FWD 5' AGAACGCACCGTTACTGAGC 3'

REV 5' ATCTCTACGGTGGCGTGTCT 3'

Fwd Tm 62.2°C

Rev Tm 62.5°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001293671 · chr3

107 Specific

FWD 5' ACTGAGCAGCACACACAGAG 3'

REV 5' GATCTCTACGGTGGCGTGTC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001293671 · chr3

107 Specific

FWD 5' CTGAGCAGCACACACAGAGT 3'

REV 5' GATCTCTACGGTGGCGTGTC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001293671 · chr3

107 Specific

FWD 5' TGAGCAGCACACACAGAGTC 3'

REV 5' GATCTCTACGGTGGCGTGTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron