apoea 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001020565 · chr19

109 Specific

FWD 5' GGCTGTAATTGTTGCGCTCG 3'

REV 5' TTCCAGAACTGATCCACGGC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001020565 · chr19

109 Specific

FWD 5' CTGTAATTGTTGCGCTCGCC 3'

REV 5' TTCCAGAACTGATCCACGGC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 103 bp

Spans 1 intron

NM_001020565 · chr19

109 Specific

FWD 5' ACCATGGTGGAGCAGAACTC 3'

REV 5' GCTCTGTGAAGCACGAGACT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' GGCTGTAATTGTTGCGCTCG 3'

REV 5' AACTGATCCACGGCCTCTTC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' GGCTGTAATTGTTGCGCTCG 3'

REV 5' GAACTGATCCACGGCCTCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' CTGTAATTGTTGCGCTCGCC 3'

REV 5' AACTGATCCACGGCCTCTTC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 97 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' CTGTAATTGTTGCGCTCGCC 3'

REV 5' GAACTGATCCACGGCCTCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' GACCATGGTGGAGCAGAACT 3'

REV 5' AAGCACGAGACTGAACCTCC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' ACCATGGTGGAGCAGAACTC 3'

REV 5' AAGCACGAGACTGAACCTCC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001020565 · chr19

108 Specific

FWD 5' AGGGACAACATCAAGGCCAC 3'

REV 5' GGCCAGTTTGGAGTGCAGAT 3'

Fwd Tm 62.2°C

Rev Tm 62.5°C

GC 55%

Product 108 bp

Spans 1 intron