apol1 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001030138 · chr2

119 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' CCTTGTCAGCGGTTACAGCA 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 109 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

119 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' CCTTGTCAGCGGTTACAGCA 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 109 bp

Spans 2 introns

NM_001030138 · chr2

119 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' ACCTTGTCAGCGGTTACAGC 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 110 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

119 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' ACCTTGTCAGCGGTTACAGC 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 110 bp

Spans 2 introns

NM_001030138 · chr2

118 Specific

FWD 5' AACAATGGGAGGACATGCCC 3'

REV 5' TCCTTCTGCTCGCTCTGAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001030138 · chr2

118 Specific

FWD 5' CAATGGGAGGACATGCCCAA 3'

REV 5' TCCTTCTGCTCGCTCTGAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

118 Specific

FWD 5' AACAATGGGAGGACATGCCC 3'

REV 5' TCCTTCTGCTCGCTCTGAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

118 Specific

FWD 5' CAATGGGAGGACATGCCCAA 3'

REV 5' TCCTTCTGCTCGCTCTGAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001030138 · chr2

118 Specific

FWD 5' AACAATGGGAGGACATGCCC 3'

REV 5' AGTCCTTCTGCTCGCTCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001030138 · chr2

118 Specific

FWD 5' CAATGGGAGGACATGCCCAA 3'

REV 5' AGTCCTTCTGCTCGCTCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

118 Specific

FWD 5' AACAATGGGAGGACATGCCC 3'

REV 5' AGTCCTTCTGCTCGCTCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

118 Specific

FWD 5' CAATGGGAGGACATGCCCAA 3'

REV 5' AGTCCTTCTGCTCGCTCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001030138 · chr2

118 Specific

FWD 5' TTGAGGGCCTCATGGATTGG 3'

REV 5' CCTTGTCAGCGGTTACAGCA 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 114 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

118 Specific

FWD 5' TTGAGGGCCTCATGGATTGG 3'

REV 5' CCTTGTCAGCGGTTACAGCA 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 114 bp

Spans 2 introns

NM_001030138 · chr2

118 Specific

FWD 5' TTGAGGGCCTCATGGATTGG 3'

REV 5' ACCTTGTCAGCGGTTACAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 115 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

118 Specific

FWD 5' TTGAGGGCCTCATGGATTGG 3'

REV 5' ACCTTGTCAGCGGTTACAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 115 bp

Spans 2 introns

NM_001030138 · chr2

117 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' GCACCTTGTCAGCGGTTACA 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001030138 · chr2

117 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' TGCACCTTGTCAGCGGTTAC 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 113 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

117 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' GCACCTTGTCAGCGGTTACA 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001030138 · chr2_KZ115043v1_alt

117 Specific

FWD 5' GGCCTCATGGATTGGTGGAA 3'

REV 5' TGCACCTTGTCAGCGGTTAC 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 55%

Product 113 bp

Spans 2 introns