appb 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_152886 · chr9_KZ115297v1_alt

119 Specific

FWD 5' CGAGAGCTTTAACCAGGCCA 3'

REV 5' TCCTCAATGTCCAGCCGAAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_152886 · chr9

119 Specific

FWD 5' CGAGAGCTTTAACCAGGCCA 3'

REV 5' TCCTCAATGTCCAGCCGAAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

119 Specific

FWD 5' CGAGAGCTTTAACCAGGCCA 3'

REV 5' CTCAATGTCCAGCCGAACCT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 118 bp

Spans 2 introns

NM_152886 · chr9

119 Specific

FWD 5' CGAGAGCTTTAACCAGGCCA 3'

REV 5' CTCAATGTCCAGCCGAACCT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 118 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

119 Specific

FWD 5' TTGCCTACGAGACCCGAGAT 3'

REV 5' GCCCAATGATCGCACCCTTA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

119 Specific

FWD 5' TGCCTACGAGACCCGAGATT 3'

REV 5' GCCCAATGATCGCACCCTTA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 135 bp

Spans 2 introns

NM_152886 · chr9

119 Specific

FWD 5' TTGCCTACGAGACCCGAGAT 3'

REV 5' GCCCAATGATCGCACCCTTA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_152886 · chr9

119 Specific

FWD 5' TGCCTACGAGACCCGAGATT 3'

REV 5' GCCCAATGATCGCACCCTTA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 135 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

119 Specific

FWD 5' TTGCCTACGAGACCCGAGAT 3'

REV 5' CCCACCATCAGCCCAATGAT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

119 Specific

FWD 5' TGCCTACGAGACCCGAGATT 3'

REV 5' CCCACCATCAGCCCAATGAT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_152886 · chr9

119 Specific

FWD 5' TTGCCTACGAGACCCGAGAT 3'

REV 5' CCCACCATCAGCCCAATGAT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_152886 · chr9

119 Specific

FWD 5' TGCCTACGAGACCCGAGATT 3'

REV 5' CCCACCATCAGCCCAATGAT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

118 Predicted

FWD 5' TTAACCAGGCCAACACCGAC 3'

REV 5' GTCCAGCCGAACCTTTGGAA 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 104 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

118 Predicted

FWD 5' CAACACCGACAACCACGTTG 3'

REV 5' TCCTCAATGTCCAGCCGAAC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 102 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

118 Predicted

FWD 5' CAACACCGACAACCACGTTG 3'

REV 5' CTCAATGTCCAGCCGAACCT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 100 bp

Spans 2 introns

NM_152886 · chr9_KZ115297v1_alt

118 Predicted

FWD 5' CCGACAACCACGTTGAACCT 3'

REV 5' ATGTCCAGCCGAACCTTTGG 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 91 bp

Spans 2 introns

NM_152886 · chr9

118 Specific

FWD 5' AAGGCTTCACAGATTCGGCC 3'

REV 5' CTGATCACGCTGAACCAGCA 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 147 bp

Spans 2 introns

NM_152886 · chr9

118 Specific

FWD 5' TTTGCCTACGAGACCCGAGA 3'

REV 5' GCCCAATGATCGCACCCTTA 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 137 bp

Spans 2 introns

NM_152886 · chr9

118 Specific

FWD 5' GGCTTCACAGATTCGGCCAT 3'

REV 5' TGATCACGCTGAACCAGCAC 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_152886 · chr9

118 Specific

FWD 5' TCGGCCATTTGTGATGACCC 3'

REV 5' TGATCACGCTGAACCAGCAC 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 132 bp

Spans 2 introns