appbp2 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_212633 · chr15

117 Specific

FWD 5' CAAGCTTTACCAGCAAGGCC 3'

REV 5' AAGCAGTGGTGTAGGAGGTG 3'

Fwd Tm 61.5°C

Rev Tm 61.3°C

GC 55%

Product 114 bp

Spans 2 introns

NM_212633 · chr15

117 Specific

FWD 5' TATTCCACGCAGAACGTGCC 3'

REV 5' GCGCTCCTCGGTTTCTTTGT 3'

Fwd Tm 62.6°C

Rev Tm 62.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' GCACTATTCCACGCAGAACG 3'

REV 5' GGCAATCGATGGCAATCTCC 3'

Fwd Tm 61.0°C

Rev Tm 61.1°C

GC 55%

Product 124 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' CTATTCCACGCAGAACGTGC 3'

REV 5' GGCAATCGATGGCAATCTCC 3'

Fwd Tm 61.0°C

Rev Tm 61.1°C

GC 55%

Product 121 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' GCACTATTCCACGCAGAACG 3'

REV 5' TCGATGGCAATCTCCTCCAG 3'

Fwd Tm 61.0°C

Rev Tm 61.2°C

GC 55%

Product 119 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' CTATTCCACGCAGAACGTGC 3'

REV 5' TCGATGGCAATCTCCTCCAG 3'

Fwd Tm 61.0°C

Rev Tm 61.2°C

GC 55%

Product 116 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' GCACTATTCCACGCAGAACG 3'

REV 5' GATGGCAATCTCCTCCAGGA 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 55%

Product 117 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' CTATTCCACGCAGAACGTGC 3'

REV 5' GATGGCAATCTCCTCCAGGA 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 55%

Product 114 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' AAGCTTTACCAGCAAGGCCG 3'

REV 5' ATCAACGCCTGGAAGCAGTG 3'

Fwd Tm 63.0°C

Rev Tm 62.7°C

GC 55%

Product 125 bp

Spans 2 introns

NM_212633 · chr15

116 Specific

FWD 5' AAGCTTTACCAGCAAGGCCG 3'

REV 5' CATCAACGCCTGGAAGCAGT 3'

Fwd Tm 63.0°C

Rev Tm 62.7°C

GC 55%

Product 126 bp

Spans 2 introns