aptx 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_214729 · chr1

120 Specific

FWD 5' CCCAGCATGAGTCACGTTCA 3'

REV 5' CTTCCCATCATGCTCCAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 2 introns

NM_214729 · chr1

119 Specific

FWD 5' AGCAGATGTGTCAGACAGCC 3'

REV 5' CCACCACGCTGTCGTCTTTA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 149 bp

Spans 2 introns

NM_214729 · chr1

119 Specific

FWD 5' TCCCAGCATGAGTCACGTTC 3'

REV 5' CTTCCCATCATGCTCCAGCA 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_214729 · chr1

119 Specific

FWD 5' CCCAGCATGAGTCACGTTCA 3'

REV 5' CACCTTCCCATCATGCTCCA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_214729 · chr1

118 Specific

FWD 5' AGATGTGTCAGACAGCCCAC 3'

REV 5' CCACCACGCTGTCGTCTTTA 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 146 bp

Spans 2 introns

NM_214729 · chr1

118 Specific

FWD 5' TCCCAGCATGAGTCACGTTC 3'

REV 5' ACCTTCCCATCATGCTCCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.5°C

GC 55%

Product 150 bp

Spans 2 introns

NM_214729 · chr1

118 Specific

FWD 5' CAGATGTGTCAGACAGCCCA 3'

REV 5' CCACCACGCTGTCGTCTTTA 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 2 introns

NM_214729 · chr1

118 Specific

FWD 5' AGTCTGTGACTCTGGGTCGA 3'

REV 5' AGTGTCGGATTCACACCCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 55%

Product 133 bp

Spans 2 introns

NM_214729 · chr1

118 Specific

FWD 5' AGATGTGTCAGACAGCCCAC 3'

REV 5' ATTACCACCACGCTGTCGTC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 150 bp

Spans 2 introns

NM_214729 · chr1

117 Specific

FWD 5' CCCAGCATGAGTCACGTTCA 3'

REV 5' ACCTTCCCATCATGCTCCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 55%

Product 149 bp

Spans 2 introns