aqr 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200464 · chr17

120 Specific

FWD 5' AAAGTGACCACAGTCGACCG 3'

REV 5' GAGACATGGCCACAACCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 121 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' GCAGCCCAGTAAAGTGACCA 3'

REV 5' AGACATGGCCACAACCAGAC 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 130 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' ACACCACAGCGACAACAGAG 3'

REV 5' AGTGGTGCTCTTCTCCTCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 125 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' ACACCACAGCGACAACAGAG 3'

REV 5' AGTGGTGCTCTTCTCCTCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 95 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' ACACCACAGCGACAACAGAG 3'

REV 5' TTCTCCTCTGTCCCAGTGGT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' ACACCACAGCGACAACAGAG 3'

REV 5' TTCTCCTCTGTCCCAGTGGT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' TCGGTCATCTCAGTCCCAGT 3'

REV 5' TCGTCTCTCGTGATGGGACA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 137 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' CCCATGTGGATGTGTCTGCT 3'

REV 5' GAGGAAGGTGCGCTCTTTCT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' AGGAGAAGAACACCACAGCG 3'

REV 5' CTCTTCAGCTGCTGTGGTGA 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns

NM_200464 · chr17

119 Specific

FWD 5' AGGAGAAGAACACCACAGCG 3'

REV 5' TCTCCTCCTTTGCTGCTGTG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 123 bp

Spans 2 introns