arsk 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001077157 · chr5_KZ115147v1_alt

110 Specific

FWD 5' GGTGAAGTAATGGCGGCTCT 3'

REV 5' TGCCTGTGTTCCATGGCTAG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

110 Specific

FWD 5' GGTGAAGTAATGGCGGCTCT 3'

REV 5' CTGCCTGTGTTCCATGGCTA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001077157 · chr5

110 Specific

FWD 5' GGTGAAGTAATGGCGGCTCT 3'

REV 5' TGCCTGTGTTCCATGGCTAG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001077157 · chr5

110 Specific

FWD 5' GGTGAAGTAATGGCGGCTCT 3'

REV 5' CTGCCTGTGTTCCATGGCTA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' TGATCTTGCTGGTGTCCCAC 3'

REV 5' GAGGTGTTGGCATTGCAACC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' TGATCTTGCTGGTGTCCCAC 3'

REV 5' GGAGGTGTTGGCATTGCAAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GATCTTGCTGGTGTCCCACA 3'

REV 5' GAGGTGTTGGCATTGCAACC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GATCTTGCTGGTGTCCCACA 3'

REV 5' GGAGGTGTTGGCATTGCAAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' TGATCTTGCTGGTGTCCCAC 3'

REV 5' GAGGTGTTGGCATTGCAACC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' TGATCTTGCTGGTGTCCCAC 3'

REV 5' GGAGGTGTTGGCATTGCAAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' GATCTTGCTGGTGTCCCACA 3'

REV 5' GAGGTGTTGGCATTGCAACC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' GATCTTGCTGGTGTCCCACA 3'

REV 5' GGAGGTGTTGGCATTGCAAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' ATGTCTGACGCCTTCGATGG 3'

REV 5' CGGACAGCAGATGGGTGAAT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' ATGTCTGACGCCTTCGATGG 3'

REV 5' CGGACAGCAGATGGGTGAAT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GCCATGTGGAGTGGACAGTT 3'

REV 5' TCCAGGCCTCCACTCGATTA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 181 bp

Spans 2 introns

NM_001077157 · chr5

109 Specific

FWD 5' GCCATGTGGAGTGGACAGTT 3'

REV 5' TCCAGGCCTCCACTCGATTA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 181 bp

Spans 2 introns

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' AGTCTACCGGTGTCACTGGT 3'

REV 5' TCAGAGGGATCAGCGAGTGA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' AGTCTACCGGTGTCACTGGT 3'

REV 5' TCAGAGGGATCAGCGAGTGA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001077157 · chr5_KZ115147v1_alt

109 Specific

FWD 5' GGTGAAGTAATGGCGGCTCT 3'

REV 5' TGTTCCATGGCTAGATCGCC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 92 bp

Spans 1 intron

NM_001077157 · chr5

109 Specific

FWD 5' GGTGAAGTAATGGCGGCTCT 3'

REV 5' TGTTCCATGGCTAGATCGCC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 92 bp

Spans 1 intron