asah2 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001007763 · chr12

119 Specific

FWD 5' TACAGGGCGGACGTGTAAAC 3'

REV 5' ATGCCATCCCAGAAAGGGTC 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' TTTCTGGAAACCCGAGGCAC 3'

REV 5' TGCCCGCGAGTCCTTTAATC 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' TGGGTGAAATCGCATCCGTC 3'

REV 5' CCATGAGCCTGTGTCGTTGT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 113 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' GGGTGAAATCGCATCCGTCA 3'

REV 5' CCATGAGCCTGTGTCGTTGT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' CTCTTGCCCTGTTGGAGGAA 3'

REV 5' GCTTTGCACTGCCATACAGC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 128 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' AGTGGGTGAAATCGCATCCG 3'

REV 5' CCATGAGCCTGTGTCGTTGT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 115 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' TGGGTGAAATCGCATCCGTC 3'

REV 5' TTTCCCATGAGCCTGTGTCG 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 117 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' GGGTGAAATCGCATCCGTCA 3'

REV 5' TTTCCCATGAGCCTGTGTCG 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 116 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' AAGGAGCTGTATGGCAGTGC 3'

REV 5' CTGTGACCCAGAGCTGGTTT 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001007763 · chr12

118 Specific

FWD 5' AAGGAGCTGTATGGCAGTGC 3'

REV 5' AAACTGTGACCCAGAGCTGG 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 143 bp

Spans 2 introns