asb12a 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001126376 · chr5

110 Specific

FWD 5' AGAACATCTTCCGCTGCTCG 3'

REV 5' ATGGTCATCATCTGCCACGG 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

110 Specific

FWD 5' AGAACATCTTCCGCTGCTCG 3'

REV 5' ATGGTCATCATCTGCCACGG 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' TGTAGCGTTCCTGTGACAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' TGTAGCGTTCCTGTGACAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' ATGGTCATCATCTGCCACGG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' TGCACTTCCTGTAGCGTTCC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' ATGGTCATCATCTGCCACGG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' TGCACTTCCTGTAGCGTTCC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' AGGCTGCCCAAAGAGTCTTG 3'

REV 5' ACCAGCGCAGTGTTCATAGG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' AGGCTGCCCAAAGAGTCTTG 3'

REV 5' ACCAGCGCAGTGTTCATAGG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' GCACTTCCTGTAGCGTTCCT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' AGCTCGGTCTCCTCTATGCT 3'

REV 5' GCACTTCCTGTAGCGTTCCT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' CCGTGGCAGATGATGACCAT 3'

REV 5' AACTGCAGGCACCTCAGATG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' CCGTGGCAGATGATGACCAT 3'

REV 5' CAACTGCAGGCACCTCAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' GGAACGCTACAGGAAGTGCA 3'

REV 5' AACTGCAGGCACCTCAGATG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001126376 · chr5

109 Specific

FWD 5' GGAACGCTACAGGAAGTGCA 3'

REV 5' CAACTGCAGGCACCTCAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 103 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' CCGTGGCAGATGATGACCAT 3'

REV 5' AACTGCAGGCACCTCAGATG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' CCGTGGCAGATGATGACCAT 3'

REV 5' CAACTGCAGGCACCTCAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' GGAACGCTACAGGAAGTGCA 3'

REV 5' AACTGCAGGCACCTCAGATG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001126376 · chr5_KZ115109v1_alt

109 Specific

FWD 5' GGAACGCTACAGGAAGTGCA 3'

REV 5' CAACTGCAGGCACCTCAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 103 bp

Spans 1 intron