asb12b 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001004009 · chr14_KZ114936v1_alt

102 Specific

FWD 5' ATACTGACCCTCGCTGCTAC 3'

REV 5' TGCTGGTGGACTTATCCCAA 3'

Fwd Tm 60.8°C

Rev Tm 60.6°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001004009 · chr14

102 Specific

FWD 5' ATACTGACCCTCGCTGCTAC 3'

REV 5' TGCTGGTGGACTTATCCCAA 3'

Fwd Tm 60.8°C

Rev Tm 60.6°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

101 Specific

FWD 5' ATACTGACCCTCGCTGCTAC 3'

REV 5' CATGCTGGTGGACTTATCCC 3'

Fwd Tm 60.8°C

Rev Tm 60.0°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001004009 · chr14

101 Specific

FWD 5' ATACTGACCCTCGCTGCTAC 3'

REV 5' CATGCTGGTGGACTTATCCC 3'

Fwd Tm 60.8°C

Rev Tm 60.0°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

101 Specific

FWD 5' ATACTGACCCTCGCTGCTAC 3'

REV 5' ATGCTGGTGGACTTATCCCA 3'

Fwd Tm 60.8°C

Rev Tm 60.5°C

GC 55%

Product 110 bp

Spans 1 intron

NM_001004009 · chr14

101 Specific

FWD 5' ATACTGACCCTCGCTGCTAC 3'

REV 5' ATGCTGGTGGACTTATCCCA 3'

Fwd Tm 60.8°C

Rev Tm 60.5°C

GC 55%

Product 110 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' CTGCAGTTGGTGTCTTCCTC 3'

Fwd Tm 59.6°C

Rev Tm 60.2°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001004009 · chr14

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' CTGCAGTTGGTGTCTTCCTC 3'

Fwd Tm 59.6°C

Rev Tm 60.2°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' GGTGTCTTCCTCCTCATCCT 3'

Fwd Tm 59.6°C

Rev Tm 60.3°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001004009 · chr14

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' GGTGTCTTCCTCCTCATCCT 3'

Fwd Tm 59.6°C

Rev Tm 60.3°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' CAGTTGGTGTCTTCCTCCTC 3'

Fwd Tm 59.6°C

Rev Tm 59.4°C

GC 55%

Product 131 bp

Spans 1 intron

NM_001004009 · chr14

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' CAGTTGGTGTCTTCCTCCTC 3'

Fwd Tm 59.6°C

Rev Tm 59.4°C

GC 55%

Product 131 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' TCCTCCTCATCCTCTTTGGG 3'

Fwd Tm 59.6°C

Rev Tm 60.4°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001004009 · chr14

100 Specific

FWD 5' CACACTGTACAGACTCGAGC 3'

REV 5' TCCTCCTCATCCTCTTTGGG 3'

Fwd Tm 59.6°C

Rev Tm 60.4°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

100 Specific

FWD 5' AGCAGTAGTGCTTCTGCTCA 3'

REV 5' TTTCCGCCGGAAGGAACTTC 3'

Fwd Tm 60.8°C

Rev Tm 62.4°C

GC 50%

Product 92 bp

Spans 1 intron

NM_001004009 · chr14

100 Specific

FWD 5' AGCAGTAGTGCTTCTGCTCA 3'

REV 5' TTTCCGCCGGAAGGAACTTC 3'

Fwd Tm 60.8°C

Rev Tm 62.4°C

GC 50%

Product 92 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

99 Specific

FWD 5' GCTACCGCGTTACATGCATT 3'

REV 5' TGCTGGTGGACTTATCCCAA 3'

Fwd Tm 60.7°C

Rev Tm 60.6°C

GC 50%

Product 94 bp

Spans 1 intron

NM_001004009 · chr14

99 Specific

FWD 5' GCTACCGCGTTACATGCATT 3'

REV 5' TGCTGGTGGACTTATCCCAA 3'

Fwd Tm 60.7°C

Rev Tm 60.6°C

GC 50%

Product 94 bp

Spans 1 intron

NM_001004009 · chr14_KZ114936v1_alt

99 Specific

FWD 5' ACACTGTACAGACTCGAGCA 3'

REV 5' CTGCAGTTGGTGTCTTCCTC 3'

Fwd Tm 60.0°C

Rev Tm 60.2°C

GC 50%

Product 133 bp

Spans 1 intron

NM_001004009 · chr14

99 Specific

FWD 5' ACACTGTACAGACTCGAGCA 3'

REV 5' CTGCAGTTGGTGTCTTCCTC 3'

Fwd Tm 60.0°C

Rev Tm 60.2°C

GC 50%

Product 133 bp

Spans 1 intron