asb13a.2 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001126110 · chr4_KZ115089v1_alt

110 Specific

FWD 5' TGCAGGAGCTCAGGTTGATG 3'

REV 5' ATCCAGAAGCAGCCTCACAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

110 Specific

FWD 5' CAGGAGCTCAGGTTGATGCA 3'

REV 5' ATCCAGAAGCAGCCTCACAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001126110 · chr4

110 Specific

FWD 5' TGCAGGAGCTCAGGTTGATG 3'

REV 5' ATCCAGAAGCAGCCTCACAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001126110 · chr4

110 Specific

FWD 5' CAGGAGCTCAGGTTGATGCA 3'

REV 5' ATCCAGAAGCAGCCTCACAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

110 Specific

FWD 5' TGCAGGAGCTCAGGTTGATG 3'

REV 5' GAAGCAGCCTCACACACTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

110 Specific

FWD 5' CAGGAGCTCAGGTTGATGCA 3'

REV 5' GAAGCAGCCTCACACACTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 93 bp

Spans 1 intron

NM_001126110 · chr4

110 Specific

FWD 5' TGCAGGAGCTCAGGTTGATG 3'

REV 5' GAAGCAGCCTCACACACTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 1 intron

NM_001126110 · chr4

110 Specific

FWD 5' CAGGAGCTCAGGTTGATGCA 3'

REV 5' GAAGCAGCCTCACACACTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 93 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

109 Specific

FWD 5' TGCAGGAGCTCAGGTTGATG 3'

REV 5' GGTGAGGGTTGGGTTGACTT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

109 Specific

FWD 5' CAGGAGCTCAGGTTGATGCA 3'

REV 5' GGTGAGGGTTGGGTTGACTT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001126110 · chr4

109 Specific

FWD 5' TGCAGGAGCTCAGGTTGATG 3'

REV 5' GGTGAGGGTTGGGTTGACTT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001126110 · chr4

109 Specific

FWD 5' CAGGAGCTCAGGTTGATGCA 3'

REV 5' GGTGAGGGTTGGGTTGACTT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

109 Specific

FWD 5' CCTGCATGGGAGGTAATGCA 3'

REV 5' AGCCTAGTGGCATTGACGTG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 172 bp

Spans 2 introns

NM_001126110 · chr4

109 Specific

FWD 5' CCTGCATGGGAGGTAATGCA 3'

REV 5' AGCCTAGTGGCATTGACGTG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 172 bp

Spans 2 introns

NM_001126110 · chr4_KZ115089v1_alt

109 Specific

FWD 5' TCTTCATGAGGCCTGCATGG 3'

REV 5' AGCCTAGTGGCATTGACGTG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 183 bp

Spans 2 introns

NM_001126110 · chr4

109 Specific

FWD 5' TCTTCATGAGGCCTGCATGG 3'

REV 5' AGCCTAGTGGCATTGACGTG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 183 bp

Spans 2 introns

NM_001126110 · chr4_KZ115089v1_alt

109 Specific

FWD 5' TCTTCATGAGGCCTGCATGG 3'

REV 5' ACATTCGAGTCGTCGGTTGG 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001126110 · chr4

109 Specific

FWD 5' TCTTCATGAGGCCTGCATGG 3'

REV 5' ACATTCGAGTCGTCGGTTGG 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001126110 · chr4_KZ115089v1_alt

109 Specific

FWD 5' AGTCTCGAGGCGTATGACCT 3'

REV 5' AGCCTAGTGGCATTGACGTG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001126110 · chr4

109 Specific

FWD 5' AGTCTCGAGGCGTATGACCT 3'

REV 5' AGCCTAGTGGCATTGACGTG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 119 bp

Spans 1 intron