ascl1b 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_131231 · chr7

109 Specific

FWD 5' AGGAGCAAGAACTGCTGGAC 3'

REV 5' CAGGACTGGTCACTTGCTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 107 bp

Spans 1 intron

NM_131231 · chr7

109 Specific

FWD 5' GGAGCAAGAACTGCTGGACT 3'

REV 5' CAGGACTGGTCACTTGCTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 106 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' TGCTGGACTTCACCACATGG 3'

REV 5' CAGGACTGGTCACTTGCTGT 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 95 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' GAGGAGCAAGAACTGCTGGA 3'

REV 5' CAGGACTGGTCACTTGCTGT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 108 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' GCTGGACTTCACCACATGGT 3'

REV 5' CAGGACTGGTCACTTGCTGT 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 94 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' GAGGAGCAAGAACTGCTGGA 3'

REV 5' GGACTGGTCACTTGCTGTCT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 106 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' GAGGAGCAAGAACTGCTGGA 3'

REV 5' AGGACTGGTCACTTGCTGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 107 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' TGCTGGACTTCACCACATGG 3'

REV 5' GTCAACGAACTCGGGTCCTT 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 146 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' TGCTGGACTTCACCACATGG 3'

REV 5' CAACGAACTCGGGTCCTTCA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 144 bp

Spans 1 intron

NM_131231 · chr7

108 Specific

FWD 5' GCTGGACTTCACCACATGGT 3'

REV 5' GTCAACGAACTCGGGTCCTT 3'

Fwd Tm 62.2°C

Rev Tm 61.7°C

GC 55%

Product 145 bp

Spans 1 intron