asf1bb 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_214757 · chr1

110 Specific

FWD 5' GGGACAGGAATTCATCCGCA 3'

REV 5' CGAGGGTTTGAGGCCAAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 129 bp

Spans 1 intron

NM_214757 · chr1

109 Specific

FWD 5' CCGACTACACACAGCTGCAA 3'

REV 5' TCTTGTCCATTGTGGCCTCC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 90 bp

Spans 1 intron

NM_214757 · chr1

109 Specific

FWD 5' AACCCGACTACACACAGCTG 3'

REV 5' TCTTGTCCATTGTGGCCTCC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 93 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' AGACCCTGAGCTACGGGAAA 3'

REV 5' TTGTGGCCTCCCAGTTGATG 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 116 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' AGACCCTGAGCTACGGGAAA 3'

REV 5' CATTGTGGCCTCCCAGTTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 118 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' CGGTCAAACCCGACTACACA 3'

REV 5' TCTTGTCCATTGTGGCCTCC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' AGACCCTGAGCTACGGGAAA 3'

REV 5' TCCCAGTTGATGTGGAAGCG 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 108 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' AGACCCTGAGCTACGGGAAA 3'

REV 5' GCCTCCCAGTTGATGTGGAA 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 111 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' AGACCCTGAGCTACGGGAAA 3'

REV 5' TCTTGTCCATTGTGGCCTCC 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 125 bp

Spans 1 intron

NM_214757 · chr1

108 Specific

FWD 5' TTCCTGCTGGCAGACACATG 3'

REV 5' TGCGGATGAATTCCTGTCCC 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 1 intron