asic1a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_214790 · chr8

119 Specific

FWD 5' ACCGACTGCGAGACTCGATA 3'

REV 5' AGTCGCTGTCAGTTTCCACC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 2 introns

NM_214790 · chr8

119 Specific

FWD 5' ACCGACTGCGAGACTCGATA 3'

REV 5' TCGCTGTCAGTTTCCACCAG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 2 introns

NM_214790 · chr8

119 Specific

FWD 5' ACCGACTGCGAGACTCGATA 3'

REV 5' GTCGCTGTCAGTTTCCACCA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_214790 · chr8

118 Specific

FWD 5' ACCGACTGCGAGACTCGATA 3'

REV 5' GCTGTCAGTTTCCACCAGGA 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_214790 · chr8

118 Specific

FWD 5' AAAGCCTATGAGGTGGCTGG 3'

REV 5' GTTTCTTGTTGGAGCAGCGG 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 148 bp

Spans 2 introns

NM_214790 · chr8

118 Specific

FWD 5' GGAAAGCCTATGAGGTGGCT 3'

REV 5' GTTTCTTGTTGGAGCAGCGG 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_214790 · chr8

118 Specific

FWD 5' ACCATCGAGCAGAGGAAAGC 3'

REV 5' AGCGGCACAACCTGTACTTC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 148 bp

Spans 2 introns

NM_214790 · chr8

117 Specific

FWD 5' AAAGCCTATGAGGTGGCTGG 3'

REV 5' AGCGGCACAACCTGTACTTC 3'

Fwd Tm 61.8°C

Rev Tm 62.3°C

GC 55%

Product 133 bp

Spans 2 introns

NM_214790 · chr8

117 Specific

FWD 5' TGGTGGAGAACTGCAACTGC 3'

REV 5' AGTCTCGCACGAGCAGTAGT 3'

Fwd Tm 62.6°C

Rev Tm 62.6°C

GC 55%

Product 140 bp

Spans 2 introns

NM_214790 · chr8

117 Specific

FWD 5' TGGTGGAGAACTGCAACTGC 3'

REV 5' GTCGCTGTCAGTTTCCACCA 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 55%

Product 122 bp

Spans 2 introns