asic4a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_214787 · chr9

115 Specific

FWD 5' GCCGGGAGATGCCAATATCT 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 61.6°C

Rev Tm 60.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_214787 · chr9

115 Specific

FWD 5' TGCCGGGAGATGCCAATATC 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 61.9°C

Rev Tm 60.9°C

GC 55%

Product 121 bp

Spans 2 introns

NM_214787 · chr9

114 Specific

FWD 5' CATGCCGGGAGATGCCAATA 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 62.1°C

Rev Tm 60.9°C

GC 55%

Product 123 bp

Spans 2 introns

NM_214787 · chr9

114 Specific

FWD 5' GCCGGGAGATGCCAATATCT 3'

REV 5' TCGTGTCAGGTTACAGGGAG 3'

Fwd Tm 61.6°C

Rev Tm 60.6°C

GC 55%

Product 121 bp

Spans 2 introns

NM_214787 · chr9

113 Specific

FWD 5' TGCCGGGAGATGCCAATATC 3'

REV 5' TCGTGTCAGGTTACAGGGAG 3'

Fwd Tm 61.9°C

Rev Tm 60.6°C

GC 55%

Product 122 bp

Spans 2 introns

NM_214787 · chr9

113 Specific

FWD 5' CAAATGCAGGATGGTGCACA 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 50%

Product 141 bp

Spans 2 introns

NM_214787 · chr9

113 Specific

FWD 5' GTGCAAATGCAGGATGGTGC 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 62.3°C

Rev Tm 60.9°C

GC 55%

Product 144 bp

Spans 2 introns

NM_214787 · chr9

113 Specific

FWD 5' GCAAATGCAGGATGGTGCAC 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 62.3°C

Rev Tm 60.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_214787 · chr9

113 Specific

FWD 5' ATGCCGGGAGATGCCAATAT 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 61.2°C

Rev Tm 60.9°C

GC 50%

Product 122 bp

Spans 2 introns

NM_214787 · chr9

113 Specific

FWD 5' AATGCAGGATGGTGCACATG 3'

REV 5' CGTGTCAGGTTACAGGGAGT 3'

Fwd Tm 60.8°C

Rev Tm 60.9°C

GC 50%

Product 139 bp

Spans 2 introns