asic4b 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_214786 · chr6

119 Specific

FWD 5' AACGAGACTACGCTGGAAGC 3'

REV 5' CGGCTGTGGCAGGTATGTTA 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' CAGGTGCAGAGAGAGTGCAA 3'

REV 5' GGACATGAGTCACCTGTGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 131 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' AGGTGCAGAGAGAGTGCAAC 3'

REV 5' GGACATGAGTCACCTGTGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 130 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' GGTGCAGAGAGAGTGCAACT 3'

REV 5' GGACATGAGTCACCTGTGCT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 129 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' AGAGAGAGTGCAACTGCAGG 3'

REV 5' GGACATGAGTCACCTGTGCT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 124 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' GAGAGAGTGCAACTGCAGGA 3'

REV 5' GGACATGAGTCACCTGTGCT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 123 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' AGGATGGTGCATATGCCAGG 3'

REV 5' GGACATGAGTCACCTGTGCT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 107 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' GAGTGCAACTGCAGGATGGT 3'

REV 5' TCTCGCAGGGACATGAGTCA 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 127 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' TGCAGAGAGAGTGCAACTGC 3'

REV 5' TCTCGCAGGGACATGAGTCA 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 135 bp

Spans 2 introns

NM_214786 · chr6

118 Specific

FWD 5' GCAGAGAGAGTGCAACTGCA 3'

REV 5' TCTCGCAGGGACATGAGTCA 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 134 bp

Spans 2 introns