asph 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001256603 · chr2

119 Specific

FWD 5' TGGGTGTCTGGACCTCTGTA 3'

REV 5' TCTCCATCGCCATCAGCATC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001256603 · chr2

119 Specific

FWD 5' TGGGTGTCTGGACCTCTGTA 3'

REV 5' AAGTCTCCATCGCCATCAGC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001256603 · chr2

119 Specific

FWD 5' TGGGTGTCTGGACCTCTGTA 3'

REV 5' CTCCATCGCCATCAGCATCA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001256603 · chr2

119 Specific

FWD 5' TGGGTGTCTGGACCTCTGTA 3'

REV 5' GTCTCCATCGCCATCAGCAT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001256603 · chr2

118 Specific

FWD 5' TGGACCTCTGTAGCTGTGGT 3'

REV 5' GTCTCCATCGCCATCAGCAT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001256603 · chr2

118 Specific

FWD 5' TGGACCTCTGTAGCTGTGGT 3'

REV 5' ACGTCAAAGTCTCCATCGCC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001256603 · chr2

118 Specific

FWD 5' TGGACCTCTGTAGCTGTGGT 3'

REV 5' CTCCATCGCCATCAGCATCA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001256603 · chr2

118 Specific

FWD 5' TGGACCTCTGTAGCTGTGGT 3'

REV 5' CGTCAAAGTCTCCATCGCCA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001256603 · chr2

118 Specific

FWD 5' TGGACCTCTGTAGCTGTGGT 3'

REV 5' AAGTCTCCATCGCCATCAGC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001256603 · chr2

118 Specific

FWD 5' CGCCAAAGCAAAGGACTTGC 3'

REV 5' ACGTCAAAGTCTCCATCGCC 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 93 bp

Spans 2 introns