ass1 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001004603 · chrUn_KN150552v1

119 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' AACCGTTCCTCCAGGAGTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 98 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

119 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' AGAACCGTTCCTCCAGGAGT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 100 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

117 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' AGCCTGAAGGAGAACCGTTC 3'

Fwd Tm 62.1°C

Rev Tm 61.5°C

GC 55%

Product 110 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

117 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' GAGCCTGAAGGAGAACCGTT 3'

Fwd Tm 62.1°C

Rev Tm 61.5°C

GC 55%

Product 111 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

117 Specific

FWD 5' CCGGAAGGACCTGATGGAAT 3'

REV 5' GGGTTCTCCAGAATGCCAGA 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

116 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' ATGAGCCTGAAGGAGAACCG 3'

Fwd Tm 62.1°C

Rev Tm 61.4°C

GC 55%

Product 113 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

116 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' GCACCTCTCGGTCCATAGTG 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

116 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' TGAGCCTGAAGGAGAACCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.8°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

116 Specific

FWD 5' TCGGAGGAAAGCATGGTGTG 3'

REV 5' GAACCGTTCCTCCAGGAGTC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 99 bp

Spans 2 introns

NM_001004603 · chrUn_KN150552v1

115 Specific

FWD 5' CCGGAAGGACCTGATGGAAT 3'

REV 5' GAAGGTGCGTGATTCTTGGG 3'

Fwd Tm 61.2°C

Rev Tm 60.9°C

GC 55%

Product 144 bp

Spans 2 introns