aste1a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001099430 · chr16

110 Specific

FWD 5' GCCATACAGACGAAGGCGAT 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001099430 · chr16

110 Specific

FWD 5' ACGAAGCAGTGAGAACGTCG 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' CAGTGAGAACGTCGAAGCCA 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' GGCTGCTACTGGGAATGGTT 3'

REV 5' CTGGCTGTAAGCATGAGCGA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' AAGCCATACAGACGAAGGCG 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' TTGTACCAGGAGCCACATGC 3'

REV 5' TGCGTTTACCAGCCAGTACC 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' TTGTACCAGGAGCCACATGC 3'

REV 5' CTCTGCGTTTACCAGCCAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 138 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' CGAAGCAGTGAGAACGTCGA 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 117 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' ACAGACGAAGGCGATGGAAG 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 91 bp

Spans 1 intron

NM_001099430 · chr16

109 Specific

FWD 5' AGCAGTGAGAACGTCGAAGC 3'

REV 5' GCCCAAAGTGTCCAGGATCA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 114 bp

Spans 1 intron