asz1 20 primer pairs found

Zebrafish

Show all (including non-specific)

NM_200230 · chr18_KZ115559v1_alt

117 Specific

FWD 5' CGTGCGCTGAGTTCTGTGAA 3'

REV 5' TGTCCAGCCAAAGCCAAGTC 3'

Fwd Tm 62.6°C

Rev Tm 62.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_200230 · chr18

117 Specific

FWD 5' CGTGCGCTGAGTTCTGTGAA 3'

REV 5' TGTCCAGCCAAAGCCAAGTC 3'

Fwd Tm 62.6°C

Rev Tm 62.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

116 Specific

FWD 5' CGTGCGCTGAGTTCTGTGAA 3'

REV 5' GTGTCCAGCCAAAGCCAAGT 3'

Fwd Tm 62.6°C

Rev Tm 62.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_200230 · chr18

116 Specific

FWD 5' CGTGCGCTGAGTTCTGTGAA 3'

REV 5' GTGTCCAGCCAAAGCCAAGT 3'

Fwd Tm 62.6°C

Rev Tm 62.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

116 Specific

FWD 5' CGGGAGCGAAGATTTCCTCA 3'

REV 5' AGGGCTTGAGCCTCTCTCTT 3'

Fwd Tm 61.6°C

Rev Tm 62.5°C

GC 55%

Product 150 bp

Spans 2 introns

NM_200230 · chr18

116 Specific

FWD 5' CGGGAGCGAAGATTTCCTCA 3'

REV 5' AGGGCTTGAGCCTCTCTCTT 3'

Fwd Tm 61.6°C

Rev Tm 62.5°C

GC 55%

Product 150 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' GATCGTCCCTCTGCCTGTAA 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 60.8°C

Rev Tm 60.8°C

GC 55%

Product 135 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' GATCGTCCCTCTGCCTGTAA 3'

REV 5' CCATGACCAACAGTTCGCTC 3'

Fwd Tm 60.8°C

Rev Tm 60.8°C

GC 55%

Product 136 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' GATCGTCCCTCTGCCTGTAA 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 60.8°C

Rev Tm 60.8°C

GC 55%

Product 135 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' GATCGTCCCTCTGCCTGTAA 3'

REV 5' CCATGACCAACAGTTCGCTC 3'

Fwd Tm 60.8°C

Rev Tm 60.8°C

GC 55%

Product 136 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' TCGTCCCTCTGCCTGTAAAG 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 133 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' TCGTCCCTCTGCCTGTAAAG 3'

REV 5' CCATGACCAACAGTTCGCTC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 134 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' CGTCCCTCTGCCTGTAAAGA 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 132 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' CGTCCCTCTGCCTGTAAAGA 3'

REV 5' CCATGACCAACAGTTCGCTC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 133 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' TCGTCCCTCTGCCTGTAAAG 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 133 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' TCGTCCCTCTGCCTGTAAAG 3'

REV 5' CCATGACCAACAGTTCGCTC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 134 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' CGTCCCTCTGCCTGTAAAGA 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 132 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' CGTCCCTCTGCCTGTAAAGA 3'

REV 5' CCATGACCAACAGTTCGCTC 3'

Fwd Tm 61.0°C

Rev Tm 60.8°C

GC 55%

Product 133 bp

Spans 2 introns

NM_200230 · chr18_KZ115559v1_alt

115 Specific

FWD 5' AAACCCTGATCGTCCCTCTG 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 61.1°C

Rev Tm 60.8°C

GC 55%

Product 142 bp

Spans 2 introns

NM_200230 · chr18

115 Specific

FWD 5' AAACCCTGATCGTCCCTCTG 3'

REV 5' CATGACCAACAGTTCGCTCC 3'

Fwd Tm 61.1°C

Rev Tm 60.8°C

GC 55%

Product 142 bp

Spans 2 introns