atg4b 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001089352 · chr6

117 Specific

FWD 5' CATCTGTGAGCTCGACCCTT 3'

REV 5' CATGGGAAGACCTCTGCAGT 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 115 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' CATCTGTGAGCTCGACCCTT 3'

REV 5' ACATGGGAAGACCTCTGCAG 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 116 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' TGCACATCTGTGAGCTCGAC 3'

REV 5' CATGGGAAGACCTCTGCAGT 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 119 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' TGCACATCTGTGAGCTCGAC 3'

REV 5' ACATGGGAAGACCTCTGCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' CCCTTCTATTGCTGCTGGCT 3'

REV 5' CATGGGAAGACCTCTGCAGT 3'

Fwd Tm 62.1°C

Rev Tm 61.4°C

GC 55%

Product 100 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' CCCTTCTATTGCTGCTGGCT 3'

REV 5' ACATGGGAAGACCTCTGCAG 3'

Fwd Tm 62.1°C

Rev Tm 61.4°C

GC 55%

Product 101 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' ATTGGCCAGTGGTATGGTCC 3'

REV 5' CAGCCATGGCATACACAACC 3'

Fwd Tm 61.8°C

Rev Tm 61.3°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001089352 · chr6

117 Specific

FWD 5' ATTGGCCAGTGGTATGGTCC 3'

REV 5' CCAGCCATGGCATACACAAC 3'

Fwd Tm 61.8°C

Rev Tm 61.3°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001089352 · chr6

116 Specific

FWD 5' GGCCAGTGGTATGGTCCAAA 3'

REV 5' CAGCCATGGCATACACAACC 3'

Fwd Tm 62.0°C

Rev Tm 61.3°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001089352 · chr6

116 Specific

FWD 5' GGCCAGTGGTATGGTCCAAA 3'

REV 5' CCAGCCATGGCATACACAAC 3'

Fwd Tm 62.0°C

Rev Tm 61.3°C

GC 55%

Product 145 bp

Spans 2 introns