atp5if1a 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001089521 · chr19

110 Specific

FWD 5' AGAGGAGGAGCGCTACTTCA 3'

REV 5' CTTGCCCTTGTGTCTGTCGA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001089521 · chr19

110 Specific

FWD 5' AGAGGAGGAGCGCTACTTCA 3'

REV 5' TCTTGCCCTTGTGTCTGTCG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' TCTGCTCAGGAGAGGCTTCT 3'

REV 5' CGAATGATCCTCCAGCAGCA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' AGAGGAGGAGCGCTACTTCA 3'

REV 5' CGGATCTTGCCCTTGTGTCT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' AGAGGAGGAGCGCTACTTCA 3'

REV 5' TCTCTCTCTGCAGACGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' TCTGCTCAGGAGAGGCTTCT 3'

REV 5' AATGATCCTCCAGCAGCACG 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' TTTGAGGATCATGGCTCGCC 3'

REV 5' AATGATCCTCCAGCAGCACG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' AGAGGAGGAGCGCTACTTCA 3'

REV 5' TGCCCTTGTGTCTGTCGATC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' TTCTTCAGCTCGCACATCCG 3'

REV 5' AATGATCCTCCAGCAGCACG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 110 bp

Spans 1 intron

NM_001089521 · chr19

109 Specific

FWD 5' TTCTTCAGCTCGCACATCCG 3'

REV 5' TGAAGTAGCGCTCCTCCTCT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 148 bp

Spans 1 intron