atraid 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001044773 · chr20

115 Specific

FWD 5' CACCAGCGATGTCGACACTA 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 61.4°C

Rev Tm 60.8°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001044773 · chr20

112 Specific

FWD 5' AAACAACACCAGCGATGTCG 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 60.8°C

Rev Tm 60.8°C

GC 50%

Product 138 bp

Spans 2 introns

NM_001044773 · chr20

112 Specific

FWD 5' AACAACACCAGCGATGTCGA 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 61.7°C

Rev Tm 60.8°C

GC 50%

Product 137 bp

Spans 2 introns

NM_001044773 · chr20

112 Specific

FWD 5' ACAACACCAGCGATGTCGAC 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 62.5°C

Rev Tm 60.8°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001044773 · chr20

112 Specific

FWD 5' CAACACCAGCGATGTCGACA 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 62.5°C

Rev Tm 60.8°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001044773 · chr20

112 Specific

FWD 5' AACACCAGCGATGTCGACAC 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 62.5°C

Rev Tm 60.8°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001044773 · chr20

111 Specific

FWD 5' ACCAGCGATGTCGACACTAT 3'

REV 5' TCACTCAGGTTGGAGATGGG 3'

Fwd Tm 60.5°C

Rev Tm 60.8°C

GC 50%

Product 131 bp

Spans 2 introns

NM_001044773 · chr20

109 Specific

FWD 5' GGCAGAGCATCATGGGAGAA 3'

REV 5' AAATCCTGGTCCGTATGGGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001044773 · chr20

109 Specific

FWD 5' GCCCATACGGACCAGGATTT 3'

REV 5' CCAGAGCACAGACGAAACCA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001044773 · chr20

109 Specific

FWD 5' GCCCATACGGACCAGGATTT 3'

REV 5' AGAGCACAGACGAAACCACC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron